![]() Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody Outstanding specificity with no cross-reactivity or background.Compatible with most blue-stained ladders.Develop your marker directly on X-ray film.Faster and easier protein detection - manual marking is no longer necessary. ![]() This makes the positioning of the marker and thus the accurate detection of proteins significantly easier. Diagenode has developed a revolutionary new antibody that specifically reacts with this blue dye, enabling direct visualization of the different marker fragments on the blot. Most prestained protein MW markers used in western blot contain fragments that are labelled with a blue dye. Learn more about this antibody Blue ladder-HRP monoclonal antibody Visualize your marker directly on film To check that the signal detection after antibody incubation is homogenous across the lines.To confirm that the electrotransfer was done equally across the lines.To verify that equal sample amount was loaded on the gel.The marker (in kDa) is shown on the left,and the position of the protein of interest is indicated on the right. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Western blot was performed on whole cell extracts (30 μg) from different cell types (lane 1: HeLa, lane 2: K562, lane 3: MCF7, lane 4: U2OS, lane 5: HepG2, lane 6: Jurkat, lane 7: NIH3T3, lane 8: E14Tg2a mouse ES cells) using the monoclonal antibody against H3. Appl Microbiol Biotechnol 102:8931–8942.Western blot analysis using H3pan monoclonal antibody Yang S, Li L, Yin S et al (2018) Single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus. Plotzki E, Keller M, Ivanusic D, Denner J (2016) A new western blot assay for the detection of porcine cytomegalovirus (PCMV). Īmeri M, Zhou EM, Hsu WH (2006) Western blot immunoassay as a confirmatory test for the presence of anti-mycoplasma hyopneumoniae antibodies in swine serum. Hedman C, Otero A, Douet JY, Lacroux C, Lugan S, Filali H, Corbière F, Aron N, Badiola JJ, Andréoletti O, Bolea R (2018) Detection of PrPres in peripheral tissue in pigs with clinical disease induced by intracerebral challenge with sheep-passaged bovine spongiform encephalopathy agent. Greenlee JJ, Kunkle RA, Smith JD, Greenlee MHW (2016) Scrapie in swine: a diagnostic challenge. (87)90076-6īhatt M, Mohapatra JK, Pandey LK, Mohanty NN, Das B, Prusty BR, Pattnaik B (2018) Mutational analysis of foot and mouth disease virus nonstructural polyprotein 3AB-coding region to design a negative marker virus. Urzainqui A, Tabarés E, Carrasco L (1987) Proteins synthesized in African swine fever virus-infected cells analyzed by two-dimensional gel electrophoresis. Current topics in veterinary medicine and animal science, vol 29. In: McNulty MS, McFerran JB (eds) Recent advances in virus diagnosis. Todd D, McNulty MS, Allan GM (1984) The use of polyacrylamide gel electrophoresis of virus RNA in the study of rotavirus infections. Kattoor JJ, Saurabh S, Malik YS, Sircar S, Dhama K, Ghosh S, Bányai K, Kobayashi N, Singh RK (2017) Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene. Kurien BT, Scofield RH (2009) Protein blotting and detection: methods and protocols. Wilson K, Walker JM, Hofmann A, Clokie S (2018) Wilson and Walker’s principles and techniques of biochemistry and molecular biology. Kurien BT, Scofield RH (2015) Western blotting: an introduction. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD et al (1985) Measurement of protein using bicinchoninic acid. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. īradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. In: Encyclopedia of movement disorders, vol 2010. Academic, Boca Raton, pp 99–117Ĭounts SE (2010) Western blot. In: Basic science methods for clinical researchers. J Mol Biol 98(3):503–517Īlwine JC, Kemp DJ, Stark GR (1977) Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. Proc Natl Acad Sci U S A 76(9):4350īurnette WN (1981) “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
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